343 MEDICAL BACTERIOLOGY 



Twenty grains each of agar-agar and peptone are dissolved in 



one liter of boiling water, and the whole made alkaline by adding 4 



ce. of a normal solution of sodium hydrate after having first brought 



the reaction to the neutral point of litmus. 



Then add, while hot, 



Sodium tauroeholate 5 grams. 



Lactose 10 grams. 



Neutral Red (5% watery solution) 10 cc 



When the solution is complete the mass is filtered through cotton, 



tubed, and sterilized in the steam sterilizer once for twenty-five or 



thirty minutes. 



D. Medium of Deigalski and Coneadi.' 



To two liters of sugar-free broth add : 



Peptone (Witte) 10 grams 



Nutrose - 10 grams 1 ,, , ... , 



„ , . , , . -, -, „ > these may be omitted 



Sodium chloride 10 grams J 



and dissolve by the aid of heat. The mixture is brought to the boil 



and sixty grams of agar-agar added, and the mixture kept boiling 



until the agar is dissolved. Then the reaction of the mass is made 



weakly alkaline to litmus by the addition of sodium hydrate (4% 



sol.) and filtered. 



This being done, a mixture of litmus solution (6%) and lactose 



(c.p.) 



Litmus sol. - - - - - 260 cc. 



Lactose - - 30 grams. 



is added while both solutions are hot, and the whole boiled gently for 

 five minutes. Then add a solution of water-free sodium carbonate 

 (10%) in the proportion of 4 cc. (this may be omitted) followed by 

 20 cc. of a fresh solution of crystal violet (Griibler's) — 0.1 gram in 

 100 cc. water— tubed, and sterilized in the steam sterilizer for 20 

 minutes on three successive days. 



All suspected cultures should be tested with typhoid blood 

 (Widal reaction). 



The typhoid organism may be isolated from the stools during the 

 first two weeks of the disease. 



Microspira comma (Asiatic cholera). 



1. Microscopal examination of "rice-water" discharges for 

 spirilla lying parallel. 



2. Culture methods. Gelatin or agar plates should be made 

 from the rice-like flakes ; other flakes should be inoculated into flasks 



1 Zeit. f . Hyg., 1902, Heft ii, p. 283. 



