348 MEDICAL BACTERIOLOGY 



a. If staphylococci alone are present search for the Pus Coccus Group. 



b. If streptococci suspfect Str. erysipelatos. 



c. If diplococci or tetracocci. 



1. Within the pus-cells test for M. gonorrhoeae or M. Weichsel- 



baumii. 



2. Free suspect Sar. tetragena. 



d. If bacilli any of the following may be searched for: 



1. B. coli. This organism is likely to be found especially in suppu- 

 rative peritonitis and diseases of the urinary organs. 2. Bact. anthracis. 

 3. Bact. pneumoniae. 4. Bact. tuberculosis. 5. Bact. leprae. 6. Bact. mal- 

 lei. 7. B. pestis. 8. Ps. aeruginosa. 9. Bact. Welehii. 10. B. oedematia. 

 11. B. tetani. 



e. Streptothrix bovis. 

 /. Ameba coli. 



Pus Micrococci. These organisms are frequently present in pus 

 and should be isolated and identified in pure cultures, as miero- 

 seopieal examinations alone will not suffice. 



Streptococcus erysipelatos. This organism is not infrequently 

 present and can be readily identified by culture methods. 



Micrococcus gonorrhoeae. Pus should be collected in a sterile 

 receptacle or spread on cover-glasses and allowed to dry. When 

 once dried it should not be wet or moistened again as this would 

 destroy the pus-cells, and hence the value of the material for diag- 

 nosis. 



a. Simple stain. 



1. Loefifler's methylen blue 3-5 minutes. 



2. Wash in water. 



3. Dry, mount in balsam and examine with -^ in. oil immer- 



sion. 



4. Look for a biscuit-shaped diplococcus within the pus cells. 

 i. Gram's method. 



1. Anilin oil gentian violet 15 minutes. 



2. Wash in water. 



3. Treat with iodine solution 2 minutes. 



4. Decolorize with alcohol. 



5. Counter-stain with eosin, % minute. 



6. Wash, dry and mount in balsam. 



7. Examine with oil immersion. 



If the gonococci be present they will be stained brown. 

 If diagnosis be' of great importance make cultures as follows : 

 a. Make 6 or more streak cultures on blood agar, or better, make 

 plates on Wertheim's medium (p. 158). Grow at 38° C. 



