362 APPENDIX A 



A. Forms endospores. 



I. Grow at room temperature. 

 1. Gelatin liquefied. 



Bad. anthracis (Koch) Mig. 



B. Without endospores. 



I. Aerobic and facultative anaerobic. 



1. Grow well on gelatin and do not liquefy it. 



a) Gram's stain negative. 



1) Gas generated in dextrose media. 



i) Gas generated in lactose media. 



Bad. aerogenes (Esch.) Mig. 



Bad. capsulatum (Sternberg) Chester, 

 ii) Little or no gas in lactose media. 



Bad. pneumonicum (Fried.) Mig. 



2) No gas in dextrose media. 



Bad. cholerae (Zopf) Kitt. 

 Bad. bovisepticum (Kruse) Mig. 

 (see also B. pestis) 



b) Gram's stain positive. 



1) Gas generated in dextrose media. 



Bad. acidi-ladici Hueppe. 

 Bad. phosphorescens (Cohn) Fischer. 



2) No gas in dextrose media. 



Bad. rhinosderomatis (Trevisan) Mig. 



2. Gelatin liquefied slowly. 



Bad. mallei (LoefSer) Mig. 

 Bad. rhusiopathiae (Kitt) Mig. 



3. Do not grow well on gelatin at room temperature. 



a) Stain with basic aniline dyes but are readily decolorized^ by 



mineral acids when stained with carbol-fuchsin. 



1) Grow well in bouillon at body temperature and stain by 



Gram's method. 



Bact. diphtheriae (LoefSer) Mig. 



Bad. pseudodiphtheriticum (Loefiler) Mig. 



2) Do not grow in bouillon or on ordinary media. 



Bad. leprae (Hansen) Mig. 



3) Growth very limited on ordinary media. 



i) Gram's stain positive. 



Bad. pneumoniae (Weichsel.) Mig. 

 ii) Gram's stain negative. 



Bad. influenzae (Pf eiffer) L. & N. 



b) Do not stain with aqueous solutions of basic aniline dyes and 



not easily decolorized by acids. 



Bad. tuberculosis (Koch) Mig. 



Bad. tuberculosis var avium (Kruse) Mig. 



II. Obligate anaerobic. 



Bad. Welchii Mig. 



Cells short or long, cylindrical, straight, without sheath, endospores present 

 or absent, motile, flagella distributed over whole body (bacilli). 



