APPENDIX A 363 



A. Form endospores. 



I. Aerobic or facultative anaerobic. 



1. Potato cultures irregularly wrinkled. 



B. vulgatus Trevisan. 

 4. Potato cultures smootli. 



B. subtilis (Ehrenb.) Cohn. 

 I. Obligate anaerobes. 



1. Kods not swollen at sporulation. 



B. oedematis Zopf. 



2. Rods spindle-shaped at sporulation. 



B. Feseri (Trevisan) Chester. 

 B. botwlinus v. Ermengen. 



3. Eods clavate-capitae at sporulation. 



B. tetani Nicolaier. 



B. Spore formation not observed. 



I. Aerobic or facultative anaerobic. 



1. Gelatin colonies roundish not distinctly ameboid. 



a) Gelatin not liquefied. 



1) Gram 's stain negative. 



1) Generate gas in dextrose media. 

 * Coagulate milk. 

 § Produce indol. 



B. coli (Escherich) Mig. 

 §§ Do not produce indol. 

 B. enteritidis Gaertner. 

 ** Do not coagulate milk. 



B. Salmonii (Trevisan) Chester. 

 B. icteroides Sanerelli. 

 ii) Gas not generated in dextrose media. 

 B. typhosus Zopf. 

 B. dysenteriae Shiga. 

 B. pestis L. & N. 



b) Liquefy gelatin. 



1) Generate gas in dextrose media. 



B. cloacae Jordan. 



2) No gas generated in dextrose media, chromogenie, pig- 



ment reddish. 



B. prodigiosus (Ehrenb.) Eluegge. 



2. Gelatin colonies ameboid or irregular. 



a) Do not liquefy gelatin. 



B. Zopfii (Kurth) Mig. 



b) Liquefy gelatin. 



B. vulgaris (Hauser) Mig. 



Cells cylindrical, straight, without sheath, endospores known in only few 

 species. Actively motile, flagella attached to the poles (pseudomonas) . 



A. Produce a greenish-bluish fluorescence in the culture media. 

 I. Gelatin liquefied. 



