372 Physiological Characters 



1. Relation to tempeeatuee : 



optimum T.°C.; limits to °C.: 



thermal death-point °C. ; time of exposure minutes; 



medium iu which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, light, disinfectants, etc.:—. 



4. Pigment production:. 



Gas production in sugar media: 



14. dextrose (1) Shake culture : 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. i8 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



i. lactose t. saccharose. 



Acid or alkali production : 



litmus milk. 



7. Reduction op nitrates: 



to nitrites ' to ammonia.. . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Chakacteeistic odor: 



11. Pathogenesis (or other special characters) : 



