376 Physiological Characteks 



1 Relation to temperature: 



optimum °C. ; limits to °C. ; 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen : 



3. Relation to othek agents, such as 



desiccation, light, disinfectants, etc. i 



4. Pigment production: 



S. Gas production in sugar media: 



u. dextrose (1) Shake culture: 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours percent. 



72 hours per cent hours per cent, 



reaction in open arm 



gas formula, H: CO2: : 



b. lactose l-. saccharose. 



Acid or alkali production: 



litmus milk . 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin — digestion of casein . 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) :. 



