380 Physiological Characters 



1. Relation to tempekature: 



optimum °C. ; limits to °C. ; 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen: 



3. Relation to other agents, such as 



desiccation, ligrht, disinfectants, etc. :- 



4. Pigment production:., 



Gas production in sugar media: 



a. dextrose (1) Shake culture: 



(2) Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent., 48 hours percent. 



72 hours per cent hours per cent. 



reaction in open arm 



gras formula, H: CO2: : 



li. lactose u. saccharose. 



AoiD or alkali production: 



litmus milk . 



7. Reduction op nitrates: 



to nitrites to ammonia.. . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) :. 



