384 Physiological uhasactees 



1. Relation to temperatuke : 



optimum °C. ; limits to °C ! 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to fkee oxygen: 



3- Relation to other agents, such as.... 

 desiccation, light, disinfectants, etc. ; 



4. Pigment production: 



Gas pboduotion in sugar media: 



«. dextrose (1) Shake culture : 



(2J Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent., 48 hours per cent. 



72 hours per cent., hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



&. lactose t. saccharose. 



6. Acid or alkali production: 



litmus milk . 



7. Reduction op nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatio 



10. Characteristic odor: 



11. Pathogenesis (or other special characters) : 



