388 Physiological Characters 



1. Relation to tesiperatuee : 



optimum °C.; limits to °C.; 



thermal death-point °C. ; time of exposure minutes; 



medium in which exposure is made 



2. Relation to free oxygen : 



3. Relation to other agents, such as 



ilesicoation, light, disinfectants, etc.:—. 



1. Pigment production:. 



Gas production in sugar media: 



u. dextrose (1) Shake culture : 



(2' Fermentation tube, growth in open arm closed arm 



rate of development: 24 hours per cent.. 48 hours per cent. 



72 hours per cent hours per cent. 



reaction in open arm 



gas formula, H: CO2: : 



b. lactose c. saccharose. 



6. Acid OR ALKALI production: 



litmus milk . 



7. Reduction of nitrates: 



to nitrites to ammonia. . . 



8. Indol production 



48 hours days . 



9. Enzyme production: 



proteolytic 



digestion of gelatin digestion of casein. 



diastatic 



10. Characteristic odor: 



H. Pathogenesis (or other special characters) :. 



