rounded by disease, apparently from :two-con- 
fluent lesions, one of which sta about mid- 
way between cuts 4 and 5, but of the opposite 
side of the limb, while the other started near 
cut 5 and on the same side of the limb. Judg- 
ing only from the size of thes¢ lesions, they 
must have originated soon after the inocula- 
tions were made. There was 2 evidence that 
any infection had started at cut|5. Cut 6 was 
uninfected. Cuts 7 and 8 showed sunken 
areas but no fans, pustules, nor other symp- 
toms of the disease. ‘Cut 9 had developed a 
girdling lesion 7 inches long with very many 
ypustules. -Cut 10.had developed a lesion 44 
inches long and 3 inches wide. Cuts .11, 12, 
18 and 14 were uninfected. .Cuts .15 and .16 
had produced confluent. girdling lesions. aggre- 
_gating 11. inches in length. This probably 
indicated that-each cut had preduced a lesion 
about 6 inches in length, as the cuts were 
about 5 inches apart. Cuts 17.and 18 were 
uninfected. ‘ 
‘The results of these inoculations may briefly 
“We “summarized as ‘follows: 
2 inoculations from typical canker on bark, 
‘both ‘successful. 
‘2 ‘inoculations from pustules on nut, both 
. successful. 
2 imoculations from ‘mycelium ‘on nut, one 
suecessful, 
10 cheeks\eut with sterile knife, none. infected. 
2 checks cut with contaminated knife, none 
infected. 
These inoculations indicate that the dis- 
ease was present on or in the nuts and burs 
collected. Although the latter were not used 
in the inoculations, the nuts and burs were 
covered with the same fungus, judging only 
from an examination with a hand lens; and, 
moreover, the nuts and burs were in contact 
when collected. 
‘Perhaps nuts infected ‘in this manner are 
not likely often to reach the market, and pre- 
sumably ‘would -be ‘unsalable either for seed 
purposes or for eating if they did reach it. 
‘Tn the ‘latter case an additional source of 
danger would be created ‘by discarding the 
diseased nuts, perhaps in a new locality far 
distant from the place where they were grown. 
In any event, the possibility of the disease at 
times being disseminated through great dis- 
tances in this manner can not be overlooked 
in summing up the evidence bearing on this 
phase of investigation. 
J. Frankuin Coins 
OFFICE OF INVESTIGATION IN FOREST 
‘PatHoLoGy, BUREAU OF PLANT INDUSTRY, 
Provipence, R. L., 
October 20, 1913 
