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Fic. 27. Other chestnut trees used in the inoculation experiments. ‘lhe tree on 
the right was inoculated April 3 in three different places. The small twig near the 
middle of the stem died on May 6, the larger one near the top on May 19. By Au- 
gust 26 the fungus had girdled the trunk at the lowest inoculation, indicated by the 
tuft of cotton, and had spread downward to the two lowest twigs. 
The experiments with cut twigs, covered with glass tubes, were repeated on the 
tree in the center, with the same result as before. Attempts were made to introduce 
the fungus into various buds and young twigs near the top of this tree without wound- 
ing the bark, but none of them were successful. 
The tree on the left was treated on April 5 in the same way, several buds and young 
twigs from one to five inches in length being covered with the fungus for some time 
under glass; but all these attempts likewise failed. The dead branch at the top was 
inoculated through a wound. 
chestnut trees within his jurisdiction appear to be dying rapidly. 
Even the young trees in the nursery there have been either en- 
tirely killed or rendered worthless by the fungus. 
