205 
An attempt was made by Mr. Merkel, * chiet forester at the 
Zoological Park, to control the disease by spraying, but I believe 
he considers the condition quite hopeless. Practically all of the 
Fic, 26. Young chestnut trees at the propagating houses used in the inocu- 
lation experiments. The tree on the right was not inoculated, being reserved as a 
check. The tree on the left shows two twigs killed. The top one was inoculated 
with the fungus March 21 and was completely girdled by April 14. By August 26 
the fungus had reached a point nearly, an inch below the junction of this twig with 
the adjoining one and had extended an equal distance up the latter, girdling it com- 
pletely. The dead twig near the base of the tree was inoculated in the same way as 
the top one, namely, by scraping off the cortex and applying the growing fungus to the 
fresh surface. The two tubes cover twigs inoculated April 3 at the ends after prun- 
ing. In both of these the fungus had reached the base of the twig by September 1. 
The tree in the center was inoculated April 3 in a fresh wound near the top of 
the main stem and in another near its base. The principal side branch, treated in 
the same way, died May 11, and by the end of August the entire stem was dead, 
including the young twig below the lowest infection, which was girdled by the fungus 
in its progress down the stem. 
* «<A deadly fungus on the American chestnut.’’ Annual Report of the New 
York Zodlogical Society 10: 97-103, ¢ figs. July 4, 1906. 
