Mar. 25, 1915 Dissemination of Chestnut-Blight Fungus 517 
nearest lesion was on a branch 13 feet to the north. The standard sugar- 
tube method of making a quantitative bacteriological analysis of air was 
employed. The bottle was refilled with 4 liters of water at intervals 
of 20 or 30 minutes, thus making the aspiration practically continuous. 
One sugar tube was generally used each day, and the quantity of air 
drawn through each tube averaged 58 liters, with a maximum of 96 liters. 
The medium employed was a 3 per cent dextrose agar, with a reaction 
of +10. Ten plates were poured for each test and were incubated 
and the colonies counted in the same way as those in the experiments 
with the water spore traps (p. 520). 
TaBLe XIV.—Summary of results of aspirator tests in 1913 at West Chester, Pa. 
» 
sty, [Number of Number of| 
Sugar oy bacteria aoa. epores of Number of 
tube Date of aspiration. Rainfall.¢| Of alr and T |Endothia| species 
No. ad yeasts aes parasitica of fungi. 
“| per liter. * | per liter. 
Inches. Liters. 
TP AUS Os 354, alent ti ciecs nota 0. 40 12 | 26. 42 4. 16 ° 4 
2 | Aug. 20........... ancl! -O 28 | 3.21 4. 28 ° 9 
3 | AUR OT chaise a8 asens ° 36 - 83 3-05 ° 9 
A |) ANG. 22s csacaes casa cee ° 48 | 2.29 5. 21 ° 7 
Be AMIS OR) cee ore ek Gets +25 68 | 31.47 | 17.65 ° 3 
6] AU 24 cid sd ca dete ° 6| 2.86 | 11.07 ° 5 
g- 24 5 
| SANA Bi hess eed h04 een ° 56 83 2 3. 96 +35 7 
8 | Aug. 26... 0c sees ° 5 4. 64 93 ° Io 
9 | AUS: 2722565 tenemos 175 47 | 2.12 8.3 ° IL 
IG fics Oss Sener eanitedalereyy ends 12 |. 3-75 5.0 -42 9 
Tr | Alig: 28. 2625 cs4a sees) ° 68 | 1.35 4. 04 ° 12 
72 || AUG 26. oe Socal otasae 40} 2.5 5.875 | 0 9 
13 es BO wise seas Gabestuness 1.10 ss I. ee 3. 2 : a 
MIPS BT orci te eaeme need ° 5 1.16 | 7. 
ie Sone itp icncon abana’ ° g2 | 1.25 | 38.37 ° II 
36) | Sept 2 ccriwiicuiykars ° 72 | 1.18 1. 87 ° 8 
17 | Sept. 3 ° 92 | 1.32 7-91 ° 12 
18. | Sept. diwcsneonce.ese! ° 56) 1.78 | 36.60 ° II 
1g | Sept. 5.....-.-....50-- ° 68 | 1.69 | 20,00 ° II 
20 |....- Oe joe eae ausiecee ote ° 4 | 16.78 6.25 ° 7 
ar | Sept. 6..........--.--. ° a 4. ze 8. 09 ° 12 
a2 | Sept.96 cccscaiwageee|eeine cee 4] 4.65 3.90 ° 13 
23 | Sept. 7 and 8 37 - 6. 25 8. 33 oe 
SEpt: 18 catcccaustarias | ee bas acy 7 - 39 4. 
a Bene 9 ° 60 16 I. 4I ° 5 
26 | Sept. I0.........--.6-- ° 76 | 2.17 6. 90 ° 10 
27 | Sept. 12.....-.-....05- ° FOE Vacca at . 1.94 ° 7 
28 | Sept. 12.2... 60... eee ele eee eee 80 | 1.50 2.58 OF bviSeate 5 
29 | Sept. 13.. 095 68 59 1.91 ° 9 
30 | Sept. 14.. ° 72 .14 I. II ° 
3r | Sept. 15 ° 72 . 62 1.18 ° #0 
32 | Sept. 16 ° 72 +41 1.25 ° 2 
33 | Sept. 17.00... eee eee epee ee eee 80 | 1.00 I. #2 o & 
34 | Sept. 18.......-..-005- 26 32 7-59 5 a ‘ 192 ey 
eee Oy cs akateacsesetlea ines 4. -15 
33 Sept. 19. . 68 96 | 1.04 4.27 ° 10 
37 | Sept. 20. . 09 76 59 I. 31 fo) Q 
8 | Sept. ar. » 43 40] 1.50 4. 50 .125 
2 fan sdOn ce saecredeur rsa . 10 28 | 1.52 6.25 . 089 Io 
4o | Sept. 22.........-+-56- =93 52 | 4.30 1. 82 ° 6 
4t | Sept. 23........------- ° 80 875 | 41.31 O-) . . Wauaetaesiess 
Average... ....0.)occeeeee 57-9 | 2.9% POF Nes seaaacss 8.8 
» 4 date of aspiration, except on Aug. 27. 
