Mar. 25, rors Dissemination of Chesinut-Blight Fungus 521 
were transferred to 3 per cent dextrose agar for further study. In gen- 
eral, it may be said that transfers were not necessary, for the colonies of 
E. parasitica are very characteristic on chestnut-bark agar at the end of 
five days, if they have had sufficient room in which to develop. It was 
only in the case of plates badly crowded with other fungi that such trans- 
fers were necessary. 
RESULTS AND DISCUSSION OF TESTS 
The water spore traps were exposed in or near the same plot of badly 
diseased chestnut trees at West Chester, Pa., which was employed for 
the exposure plates previously reported. Six different stations were 
selected for the location of water spore traps at distances. varying from 
15 to 389 feet from the nearest blight lesions, although Station V was 
404 feet from the nearest probable source of spores. More detailed in- 
formation in regard to these stations is given in Table XVI. 
TABLE XVI.—Relation of water spore-trap stations to diseased chestnut trees in 1913 at 
West Chester, Pa. 
Trap station | Distance and direction from station | Position of station with reference to diseased chest- 
No. to nearest lesions. nut trees. 
Disasvesles 25 feet west and east........ Surrounded by 15- to 18-year-old coppice. 
TDs aiweasdts 1s feet southwest; 19 feet Do. 
north. 
5 i 9 Mere so feet north; 50 feet west..| In cornfield 50 feet from coppice. 
EV vavereveieys 383 feet northwest.........] Across cornfield from coppice. 
398 feet north.............. Across open field from a single tall tree. 
NV swoseeass 237 feet northwest.........| Across pasture from a single tall tree. 
265 feet south. ..........--. Do. : 
317 feet northeast.......... Across cornfield from a single tall tree. 
4o4 feet east... ......5 0. eee Across cornfield from coppice. 
Wiicieive 389 feet south..........-.+- Across hayfield from older forest. 
TapLE XVII.—Summary of the tests with water spore traps in 1913, at West Chester, Pa. 
Total number | Number of 
Trap sta- . Date of spores of 
esa ti bey No. Culture Nos. Period of exposure. ‘cultures: a Egan Ea do thia 
I I 227-4234 | Aug. 30 to Sept. 4..] Sept. 5..| rz, 215 ° 
2 II pee wane a cc (o ore rier ..-do..... 13, 868 ° 
3 IIL | 4243-4250 | Aug. 31 to Sept. 4..|...do..... 23, 239 ° 
4 III | 4821-4835 | Sept. 4 to Sept. 8...] Sept.1o..} 1, 236 ° 
5 I | 4906-4920 | Sept. 4 to Sept. 13..} Sept. 16..] 6, 375 (?) ° 
6 I | 4951-4960 | Sept. 13 to Sept. 18.]...do..... 10, 666 1, 749 
7 II | 4967-4976 |..--- dO p.5-ties seater ...do..... 10, 583 3, 507 
8 III | 4983-4992 |----- Or iigihs oe eee ...do..... 7, 886 2,113 
9 I | 5303-5312 | Oct. 13 to Oct. 20... OCt Bisiecloi see acrenirees ° 
10 VI | 5313-5322 | Oct. 20, a. m.—p. m.].. .do..... 1, 666 30 
II IV | 5345-5354 | Oct. 19 to Oct. 22.. Oct. 23. .Jec-ceeeeeees ° 
12 Vii 5355-5304 |..--- (a eae apo GOv esx sbavictrawaiae 8 
13 I | 5371-5380 | Oct. 22 to Oct. 27.. Oct. 28...| 22, 339 37 
14 IV | 5381-5390 |----- Os ie cia eases ...do..... 13, 148 380 
15 V| 5391-5400 |.--.- do... ..eseeeee wep MO e-oee8 4, 591 820 
16 VI | 5401-5410 |...-- clo eee wan Osea. 113, 203 431 
17 I | 5411-5420 | Oct. 27 to Nov. 10. .| Nov. 11. .| Numerous. o 
18 Vi} 5421-5430 |..--- OO ick disis eavtnvecs ...do.....| Numerous. ° 
19 VI} 5431-5440 |. +--+ do.....+ sean ..do..... Numerous. ° 
