ENDOTHIA PARASITICA AND RELATED SPECIES. 37 
have grown the organisms on sterile twigs of many species and on 
liquid media. 
As stated above, the various species of Endothia are distinguish- 
able on any medium tested. White corn meal in flasks has, however, 
been most used by the writers in identification work and for keeping 
stock cultures. All the species grow readily on this medium and may 
be determined with certainty within 10 days under ordinary con- 
ditions of growth. In addition, the medium is cheap, easily pre- 
pared, and does not dry out so quickly as agar media in tubes, so 
cultures may be kept alive much longer without transfers. Almost 
equally good for purposes of identification are rice and oatmeal in 
flasks, corn-meal agar, and potato agar. 
The distinguishing characteristics of the various species in culture 
have been described rather fully in the previous publication and may 
be briefly summarized, as follows: 
CULTURES ON CORN-MEAL AGAR (UNSLANTED TUBES). 
Corn-meal agar proved the best agar medium for the production 
of pycnospores and showed constant differences in the cultural 
characters of the various species. The most characteristic differences 
appeared in cultures from six to eight weeks old on unslanted 
tubes. (See Pl. XX, figs. 2 to 7.) 
Endothia gyrosa at this age showed a rather abundant, felty white myce- 
lium, flecked with capucine buff, but there were no pyenidia. In older cul- 
tures small pycnospore threads were sometimes produced. Usually before the 
cultures were 10 days old the medium was changed to a delicate lavender 
just below the mycelium, and below this to a light olive green. A few days 
later the lavender disappeared and the green deepened to olive green. 
Endothia singularis grew more slowly than any other species. Within 
three weeks, however, the mycelium covered the entire surface. It was 
smoother than #. gyrosa and nearly white, with raw umber spots where the 
mycelium touched the glass. The medium was changed to a light hellebore 
green one-half inch below the top. 
Endothia filuens, as pointed out in the previous paper, produced an abundant 
deep-chrome mycelium, with usually one or two rather small pycnidial pus- 
tules. 
Endothia fluens mississippiensis produced a scant surface growth of my- 
celium, between cadmium yellow and raw sienna in color. The upper one-half 
centimeter of the agar became reddish orange. The pycnidial pustules were 
more numerous than those of H. flwens, but smaller and more scattered than 
those of E. parasitica. 
Endothia longirostris at the end of six weeks had a scant, webby, orange, 
aerial mycelium growing against the glass. Mycelium on the surface of the 
medium was very scant, orange to cadmium yellow in color, with scattered tiny 
xanthine orange to orange spore masses. The color of the agar changed to 
medal bronze just beneath the mycelium, shading into orange citrine below. 
Endothia tropicalis at the end of six weeks showed a thinly felted mycelium, 
white to capucine orange, with numerous small, scattered pycnidial pustules. 
The ring of mycelium against the glass was light orange yellow, as contrasted 
with white in H#. parasitica. 
