ENDOTHIA PARASITICA AND RELATED SPECIES. 39 
E. tropicalis covered the entire surface with a thin layer of surface mycelium, 
considerably darker in color than when one week old. 
Endothia perasitica.—At the end of three or four days at room temperature 
this showed a short, fluffy, white, aerial growth along the streak. The surface 
of the mycelium was orange by transmitted light, while by reflected light it was 
between raw sienna and antique brown at the sides. Within six days the 
mycelium, especially at the base of the agar slant, took on a peculiar metallic 
“brassy ” appearance, due apparently in part to the character of the mycelium 
and in part to the minute water drops scattered over the surface. This 
portion of the culture was light orange yellow by reflected light and orange 
by transmitted light. This metallic appearance has been found to be the 
most constant and reliable distinguishing character of H. parasitica on potato 
agar. In 12 to 14 days small pycnidial pustules appeared in the upper portion 
of the tubes, and the agar just below the mycelium became warbler-green, 
changing later to olive green. 
CULTURES ON CORN MEAL (IN 100 C. C. ERLENMEYER FLASKS). 
Endothia gyrosa.—Mycelial cultures one week old showed a growth of rather 
compact mycelium covering nearly one-half the surface of the medium. The 
mycelium was ochraceous buff near the point of inoculation, shading into 
white at the margin. There was no discoloration of the medium and no spore 
masses were seen. 
Cultures of the same kind one month old showed an abundant, rather thick 
growth, having the surface mostly covered with somewhat irregular tubercular 
masses, suggesting immature pycnidial stromata similar to those found in 
#. radicalis, but smaller and producing no spores. The surface of the culture 
was capucine buff, that of the tubercles honey yellow to Isabella. The dark 
color was apparently due in part to numerous superficial water drops. A por- 
tion of the medium was changed to perilla purple. 
Endothia singularis.—Mycelial cultures one week old covered only one-third 
of the surface. The growth was mostly white and fluffy, with ochraceous buff 
near the center. 
At the end of one month the growth had entirely covered the surface. The 
mycelium varied in color from cadmium orange to capucine buff, the color being 
distributed over the surface in patches. The corn meal was changed to perilla 
purple near the center. No spores were produced. 
H. singularis was readily distinguishable from FH. gyrosa, which it resembled 
more closely in culture than any of the other species, by the rate of growth and 
the color and nature of the surface of the mycelium. ZL. singularis grew more 
slowly than ZF. gyrosa, was rather brighter in color (cadmium orange), and 
the surface of the mycelium was decidedly more even, lacking the tubercular 
masses characteristic of #. gyrosa. 
Endothia fluens.—Cultures at the age of one week showed a growth of 
loose, fluffy mycelium covering one-half of the surface of the medium. The 
mycelium was deep chrome to light orange yellow at the point of inoculation, 
passing through perilla purple and light pinkish lilac and fading into white at 
the margin. Occasionally the medium was changed to perilla purple near the 
center. No spores were present. 
Cultures one month old showed a compact growth, with a nearly smooth 
surface. The color ranged from light cadmium to empire yellow. The whole 
mass of the medium was perilla purple. Spore masses were rarely present at 
this stage, but shortly afterwards a few large erumpent stromata were formed, 
which extruded spores in thick masses. 
