ENDOTHIA PARASITICA AND RELATED SPECIES. 41 
(Pl. XXI, fig. 1) in field work when fructifications of the species 
are wanting or doubtful. 
Aside from the differences in color, the most conspicuous and 
important characteristic of these fungi in corn-meal cultures is 
found in the fructification. Clinton (18, pl. 26) has already men- 
tioned and illustrated similar differences in cultures of these organ- 
isms on agar in Petri dishes. In Endothéa parasitica the pyenidia 
and spore masses are small, numerous, thickly scattered, and em- 
bedded in the mycelium. £. fluens, on the other hand, forms few, 
large, erumpent stromata, with spores extruding in thick, elongated 
masses. £’. tropicalis closely resembles EZ. parasitica in number, size, 
and arrangement of pycnidia and spore masses, but differs in color 
of mycelium. £. fluens mississippiensis appears somewhat inter- 
mediate between £’. parasitica and E. fluens in regard to the character 
and abundance of the pycnidia and in color of the growth. These 
peculiarities have been very uniform and constant in all the cultures 
on this medium and if they could be coordinated with regular mor- 
phological differences in nature would justify the separation of this 
form as a species. (See Pls. XIX and XX.) 
CULTURES ON LIQUID MEDIA (IN 100 Cc. C. FLASKS). 
Some difficulty was experienced at first in growing the species of 
Endothia satisfactorily on a liquid medium. Abundant growth was 
obtained on a medium suggested by Dr. Mel. T. Cook. This is a 
modification of the liquid medium No. II as given by him (19). 
Cook’s liquid medium, No. II, is prepared as follows: 
Into 500 c. c. of distilled water put 15 grams of glucose and 20 grams of 
peptone steamed at 100° C. for three-fourths hour; into another 500 ¢. ce. of 
distilled water put 0.25 gram of dipotassium phosphate and 0.25 gram of 
Magnesium sulphate, steamed for 20 minutes; filter both 500 ¢. c. into same 
receptacle, steam 10 minutes, put into flasks, about 30 ¢ c. in each flask, and 
autoclave. 
All species grew readily on this medium, Endothia parasitica even 
producing pycnospores. At the end of one month’s growth the sev- 
eral species were readily distinglished on this medium and may be 
briefly described as follows: 
Endothia gyrosa.—Growth scanty; did not form a continuous mat, but re- 
mained in small bunches, giving an almost flocculent appearance. The mycelium 
appeared white when removed from the culture solution, but the solution itself 
was honey yellow. 
Endothia singularis—Growth even less abundant than H. gyrosa; formed 
small brown knots against the glass. Mycelium buff, and the medium was 
changed to honey yellow. 
Endothia fiuens—Growth somewhat more abundant and less closely matted 
than H. parasitica, entirely submerged ; mycelium white; liquid unchanged in 
color. 
