56 BULLETIN 380, U. S. DEPARTMENT OF AGRICULTURE. 
diameter, which agreed in every respect with cankers produred on 
varieties of Japanese chestnuts in this country (Pl. XXIT). 
Other specimens in this collection showed well-developed perithecia 
and ascospores. The ascospore measurements made at the time, as 
well as the cultures of the Chinese fungus and the inoculation experi- 
ments on C. dentata, are described in the previous paper by the 
writers (76, p. 296). 
Shortly after this first series of inoculations was made subcul- 
tures of the Chinese material were sent. to several investigators who 
had been studying the chestnut-bark disease, in order that the 
Endothia from China might be tested as soon as possible under 
American conditions by inoculations at various points throughout 
the known range of the disease. 
A series of inoculations was made by Prof. J..Franklin Collins at 
Martic Forge, Pa., on July 14, 1913, using American and grafted 
Paragon and grafted Japanese chestnut trees. Another series of 
inoculations, 56 in number, was made at the same locality Septem- 
ber 10, 1913, by Dr. Caroline Rumbold on grafted Paragon chest- 
nuts. Twenty inoculations were made on native chestnuts at Ander- 
son, Pa., October 2, 1913, by Dr. F. D. Heald and R. A. Studhalter. 
Inoculations with the Chinese Endothia were made at Leesburg, Va., 
on both Castanea dentata and C. pumila by G. Flippo Gravatt and 
J. T. Rogers, August 16, 1913. 
All these investigators made duplicate inoculations with American 
material, and all agreed that the Chinese fungus was identical in its 
effects on the-host with the American chestnut-blight fungus. Dur- 
ing the season of 1914 numerous inoculations with material from 
China were made by the writers at various points in New Hamp- 
shire, Massachusetts, Connecticut, New York, Delaware, and Mary- 
land, while others have been made in Rhode Island by Prof. Collins 
with the same results. 
ADDITIONAL CHINESE SPECIMENS. 
Since the publication of the previous paper (76) additional speci- 
mens of Z’. parasitica from China have been received from Meyer; 
one collected at Changli, Chihli Province, China, October 13, 1913, 
by Mrs. Mary S. Clemens; a quantity of material collected by Meyer 
himself in the village of Tachingko, near Taianfu, Shantung, China, 
March 21, 1914; and another collected by him at Yatyeko, Shensi, 
China, September 2, 1914. A few cankers have also been sent by 
Meyer, collected by him at Shihbonshan, near Hangchow, Chekiang 
Province, China, June 26, 1915. The label on this specimen bears 
the further comment, “very destructive in this locality.” Cultures 
have been made from all these specimens and have invariably proved 
to be identical with cultures of Z. parasitica found in this country. 
