58 BULLETIN 380, U. S. DEPARTMENT OF AGRICULTURE. 
of the fungus. There is apparently but oné other requirement that could be 
made according to the strictest pathological canons to perfect the proof in 
this case, and that is the production of typical ascospores of EH. parasitica 
on the lesions produced by the inoculations. 
The last requirement has now been fulfilled. Specimens collected 
February 15, 1915, from inoculations made September 20, 1918, on 
chestnuts in Virginia, near Point of Rocks, Md., with Chinese ma- 
terial, show perithecial stromata with typical ascospores of EF’. par- 
asitica, thus completing the evidence. 
DISCOVERY OF ENDOTHIA PARASITICA IN JAPAN. 
More than two years after his original discovery of E'ndothia 
parasitica in China (June 3, 1913), Meyer also discovered the fungus 
in Japan. A brief account of his discovery has already been pub- 
lished by the writers (78). It may be sufficient here to state that fol- 
lowing the discovery of Endothia parasitica in China the writers 
endeavored by correspondence to obtain the fungus from Japan. 
While not successful in obtaining E'ndothia parasitica, the writers 
did receive several specimens of fungi, including species of Endothia 
on species of Castanea. These, together with’ several specimens of 
fungi found on chestnut nursery stock from Japan, make it clear 
that there are in that country several Pyrenomycetes other than 
Endothia parasitica more or less parasitic on Castanea. 
Meyer first discovered the chestnut-blight fungus in Japan at 
Nikko, September 17, 1915, on wild trees of Castanea crenata Sieb. 
and Zuce. A photograph of the trees from which he collected speci- 
mens of E'ndothia parasitica is shown in Plate XXIII, figure 1, and 
a branch from which the diseased material brought to the United 
Sates was taken is shown in Plate XXIII, figure 2. 
Shortly after Meyer’s arrival in Washington in December, 1915, 
the specimens collected at Nikko were turned over to the writers for 
study. Examination at once showed cankers and mycelial fans 
typical of E'ndothia parasitica. The material also contained typical 
pycnospores and ascospores of the fungus. Cultures made from 
single ascospores on various culture media proved to be identical 
with those of H'ndothia parasitica found in this country and in China, 
thus establishing beyond question the identity of the fungus. 
Meyer’s observations as to the resistance of the Japanese chestnuts 
to this disease are of great interest. He states that the trees vary 
considerably as regards their power of resistance, but that in general 
the Japanese chestnut is even more resistant to Endothia parasitica 
than is the Chinese chestnut (Castanea mollissima). 
As announced in the same publication (78), Endothia parasitica 
was collected by Dr. Gentaro Yamada at Morioka, northern Japan. 
These specimens, which show typical cankers as well as ascospores of 
the fungus, were received by the writers on January 8, 1916. 
