62 BULLETIN 380, U. S. DEPARTMENT OF AGRICULTURE. 
ENDOTHIA SINGULARIS. 
The material of Endothia singularis distributed by Sydow as Calo- 
pactis singularis was on Quercus gambellit Nutt. The writers have 
seen abundant material on this species as well as specimens on Q. 
utahensis (A. DC.) Rydb., Q. leptophylla Rydb., and Q. nitescens 
Rydb. Specimens on the latter two hosts were sent by Bethel, who, 
in a letter, reports finding this species also on Q. pungens Liebm. 
All of these species except Quercus leptophylla are chaparral- 
forming shrubs growing at an elevation of 4,000 feet or more. 
There is at present no evidence that the fungus is parasitic on any 
of the species. 
Inoculations with the mycelium of E’ndothia singularis were made 
on Fagus and on Quercus alba, Q. velutina, Q. rubra, and Q. palustris, 
as well as on Q. dicifolia on Overlook Mountain in the Catskills. 
No growth has, however, been noted in any case. 
ENDOTHIA FLUENS. 
When these investigations were commenced, the writers thought 
that the Endothia found in Europe might be the same as E'ndothia 
parasitica found in America. Inoculations were accordingly made 
in Maryland during October, 1912, with cultures from material col- 
lected on the chestnut by the senior writer at Stresa, Italy, and 
Etrembieres, Switzerland, using material of &. fluens sent by P. J. 
Anderson from Pennsylvania; also material of that species and of 
E. parasitica collected in Virginia as checks. In this case, as in all 
others where no special mention is made of the method, inoculations 
were made by cutting through the bark to the wood with a sharp 
knife. The inoculating material was then inserted with a freshly cut 
twig and the wound tied up either with cord or rubber bands. If 
cord was used it was cut away within two to four weeks. The rub- 
ber bands became loosened by exposure to the weather within about 
the same time. 
Inoculations were made with all the above material on sprouts of 
Castanea dentata and Quercus prinus. The results are summarized 
in Table IV. 
TasLe IV.—Inoculations of Endothia in Maryland in October, 1912. 
Number | Number 
Fungus. Host inoculated. ofinocu- | showing 
7 lations, | growth. 
Endothia parasitica... 2... ccs. cocucecneasscncace Castanea dentata............ $2 28 
DDO) cjaisttisiscimnenvies suniercameranoro a cummeenones Quercus prinus.............+ 6 0 
E. fluens 
European... Castanea dentata.. 14 u 
SA Mer Canes cicscdtonentiontmeds meneame scedeasan WO eecccscseane 26 v2) 
DOs: senaiciean eecieainnan imate sess Quercus prinus.... 12 9 
