64 BULLETIN 380, U. S. DEPARTMENT OF AGRICULTURE. 
and Vitis sp. Of these, Acer rubrum and Carya glabra gave numerous small 
pycnidia. 
On July 10 the following hosts were inoculated at Woodstock, N. Y., with 
E. fluens from Europe: Acer rubrum, A. penmnsylvanicum, Carya ovata, Corylus 
americana, Fraxinus americana, Hamamelis virginiana, Kalmia latifolia, Popu- 
lus grandidentata, Prunus serotina, Rhus glabra, Salix sp., Sassafras variifo- 
lium, and Syringa vulgaris. Each host was inoculated in six or seven places, 
but all failed to develop except two inoculations on Acer pennsylwanicum and 
one on Corylus americana. 
The results cited above are so largely negative that they prove very 
little except that the European strain shows no special affinity for 
these hosts in America. 
ENDOTHIA FLUENS MISSISSIPPIENSIS. 
Only five collections of Endothia fluens mississippiensis have thus 
far been made, three on Castanea dentata and two on Quercus sp. 
From the results of the inoculations its host relations appear very 
similar to those of E'. fluens. The results are: shown in Table V. 
TaBLe V.—Inoculations with Endothia fluens mississippiensis on Castanea and 
Quercus. 
Number | Number 
Source of culture, Host inoculated. Date. of inocu- | showing 
lations. | pycnidia. 
CASTATIOR viaieisinnisiciersassieisicinloiniestaparcraiacanemeie Jan. 20,1912 8 8 
Do... ii do... ---| May 8,1913 4 4 
Do... ats mie estan GOe ec eesins 4 4 
Do... we a isorars GO. cseiesied 9 7 
Do... t Apr. 18,1914 12 10 
QUCRCUS se sieemaiisceeeweeeecseteeesn GOs wasecie-taciienssnates faces (ooo 12 10 
The inoculations of January 20, 1912, showed no signs of growth 
until early in May, when the first signs of pycnidium formation were 
observed. The inoculations with Endothia fluens mississippiensis 
made May 8, 1913, showed within three weeks discolored areas near 
the cut which were larger than those about the check cuts. On July 
25, 1913, all of the inoculations of Z. fluens mississippiensis marked 
“successful” showed the beginnings of pycnidium formation. By 
August 80, 1913, they were producing pycnospores, which when cul- 
tured proved to be £. fluens mississippiensis. 
Inoculations were made in April, 1914, for the purpose of com- 
paring the material collected on oak with that collected on chest- 
nut. No difference was detected, and there was no indication of active 
parasitism. This form behaved in this respect exactly as did the £. 
fluens from Virginia both on Castanea dentata and Quercus prinus. 
A series of inoculations parallel to that made with Z. fluens was 
made with E. fluens mississippiensis. The same hosts were used, 
and in most cases the dates and places of the inoculation were the 
same. The results of all that showed any growth are given in 
Table VI. 
