ENDOTHIA PARASITICA AND RELATED SPECIES. 65. 
TasLeE VI.—Inoculations with Hndothia fluens mississippiensis on Acer and 
Carya. 
" Number | Number 
Location. Host. ofinocu-| showing 
lations. | pycnidia, 
Acer rubrum. ....-.-0---0+- 6 3 
Carya glabra. . 6 2 
Ae DO saiecesrsra2 6 iL: 
.| Acer rubrum... ss 1 1 
-| Carya glabra. .......-.-...-- 2 2 
As in Endothia fluens the growth was confined to the injured tis- 
sues, and there was no evidence of parasitism. 
ENDOTHIA TROPICALIS. 
The material of Endothia tropicalis from which the writers se- 
cured their cultures, was collected by T. Petch in Ceylon. As the 
species of Endothia in the Northern Hemisphere are chiefly on 
members of the Fagacez, Petch’s statements with regard to hosts 
are of considerable interest. In a letter of March 6, 1914, he writes: 
We have no Fagacer native in the island. We have introduced various 
species of Quercus and Castanea, but subsequent to Thwaite’s discovery of 
this fungus. I do not think there can be any doubt that the fungus is native 
to Ceylon * * * 
Of the speciments now sent * * * those in the packet * * * are 
from a tree which was producing shoots from the base. This tree is Elaeocar- 
pus glandulifer Mast. From the bark and habit, I believe that all my “ finds” 
of Endothia have been on this species. 
In the accounts, of the American chestnut disease, I notice that several 
authors speak of “cankers,” and give their rate of growth. I never see 
“cankers” (Krebs) on the Ceylon trees. The bark appears to die regu- 
larly and smoothly from above downward, and is quite unbroken except for 
the minute cracks through which the stromata emerge. 
Inoculations—As already noted, ascospores of E'ndothia tropi- 
calis resemble those of /. parasitica even more closely than do those 
of E. fluens. This fact, together with its similarity on culture media. 
and its oriental origin, led the writers to fear possible parasitic 
tendencies. 
Inoculation experiments were accordingly made only on the chest- 
nut and under carefully guarded conditions. In all, about 30 
inoculations were made on 2-inch chestnut sprouts, using the methods 
described for other species. 
Of 25 inoculations made in May and June, practically all had de- 
veloped a few pycnidial stromata by October 20. These stromata 
were a somewhat brighter orange than those of EZ. fluens or B. fluens 
mississippiensis, and the spores when cultured produced typical EB. 
tropicalis. In no case, however, was there any evidence of parasitism. 
43737°—Bull. 880—17-—_5 
