ENDOTHIA PARASITICA AND RELATED SPECIES. 67 
The specimen collected by Besley on Quercus prinus showed the fan- 
shaped mats of mycelium typical of Z. parasitica on Castanea spe- 
cies. The fungus had apparently girdled the tree. The specimen on 
Quercus alba, collected by Detwiler, was similar to one on Quercus 
prinus in appearance and came from a dead tree which had appar- 
ently been killed by the growth of the fungus. The specimens on 
Acer sp. and on Quercus alba were received in the spring of 1914, 
and cultures isolated from them were used in making inoculations 
for the purpose of determining whether the fungus had either lost 
or gained in virulence by passing through other hosts. 
INOCULATION EXPERIMENTS. 
The cultures secured from Acer and Quercus, together with one 
made from Castanea at about the same time, were inoculated into 
three separate sprouts of Acer rubrum, Castanea dentata, and Quer- 
cus primus. The sprouts chosen were of nearly the same size, 2 
inches in diameter, and similarly situated, and each was inoculated 
in five places, with two check cuts above. The inoculations were 
made the usual way on March 31, 1914, and were examined at least 
once a month during the summer. 
None of the inoculations on Quercus produced any growth what- 
ever. On Acer the inoculations with the culture from Quercus all 
failed to develop; one of the inoculations with the culture from 
Acer showed a few pycnidia, while four of the inoculations with 
material from the chestnut developed a few pycnidia. On Castanea 
the three series of inoculations were almost identical, every inocula- 
tion producing a typical canker. 
Of course, these inoculations are too few to be conclusive, but it 
is evident that there was no decrease in virulence on the chestnut 
in passing through Acer or Quercus and that no particular affinity 
for either Acer or Quercus was gained. On-the maple, in fact, the 
culture direct from chestnut produced the most growth. 
In addition to those listed above, numerous inoculations were made 
in order to determine whether E'ndothia parasitica had any parasitic 
tendencies on other deciduous hosts. 
These inoculations were all made during the spring of 1914 by 
the usual method of cutting well through the bark and inserting 
mycelium and spores from a pure culture, usually on corn meal. 
The wounds were then wet, some bound with wet on others with 
raffin paper, and about half were left unwrapped. 
ee inoculations were made on April 4 in Maryland on 
Alnus rugosa, Betula nigra, Carpinus caroliniana, Fagus grandifotia, 
Kalmia latifolia, Liriodendron tulipifera, and Liquidambar styraci- 
flua, none of which developed. Inoculations were also made on April 
