434 CONNECTICUT EXPERIMENT STATION REPORT, IQI2. 
parasitica will grow, in higher per cents. of tannic acid and give 
a more evident development of mycelium than E. gyrosa. The 
details of this experiment are given in the appended table. 
aa Grew. Total. Failed. 
o. — 
as Name. sie] s 4 ps, e ea s aa 
aé Sle) &laleal 2 | ss | a | &s 
4 Endothia gyrosa............. 6 |15 {10 | o | o |] 3r | 59.6)| 21 | 40.4 
4? |r, gyrosa var. parasitica...... 18 |23| 2° Oo} o |] 43 | 97.6], 1 24. 
64, Endothia gyrosa...... hte sachs r/15| 9/2] 4 || 31 | 59.6|| 21 | 40.4 
E. gyrosa var. parasitica...... 29/13} 3 | O| © || 44 |T00.0)| © 0.0 
8¢ Endothia gyrosa............. o| oO] 9g |13 | 7 |} 29] 55.7] 23 | 44-3 
E. gyrosa var. parasitica...... 15/14] 5 | 3] © || 37 84.1]| 7 | 15.9 
10% Endothia gyrosa............. o | oO} o /20 | 6 || 26 | 50.0]| 26 | 50.0 
Endothia gyrosa var. parasitica|| 2 |15 | 8 |13 | o |] 38 | 86.3]} 6 | 13.7 
INOCULATION EXPERIMENTS. 
General Conditions, etc. These experiments were undertaken 
primarily to determine the parasitic tendency of Endothia gyrosa 
as compared with that of the variety parasitica. That the latter 
could produce cankers when inoculated into chestnuts had been 
abundantly proved by the work of Murrill and others. With 
most of our inoculations both the species and the variety were 
used at the same time, and checks were also included. Nearly 
all these inoculations were made from artificial cultures, and 
usually only with conidial spores. Ordinarily a small slit in the 
bark was made with a sharp scalpel, spores from the cultures 
were introduced on a needle, the wound covered with moist 
cotton, and then bound with paraffine paper or bicycle tape. 
After several weeks the covering was removed. The checks 
were treated in the same way, except that no spores were intro- 
duced into the wound. 
In this way there were inoculated two- to three-year-old 
seedling chestnuts, four- or five-year-old chestnut sprouts, and 
two-year seedling oak at the Station Farm at Mount Carmel; 
six- to eight-year-old slow-growing chestnut seedlings at the 
Station forestry plantation at Rainbow; and two- to four-year- 
old oak sprouts in a waste lot at Highwood. The tables which 
follow give the data for all inoculations, since there are factors 
