562 BULLETIN 347 
interwoven, septate, heavy-walled hyphe running longitudinally with 
the long axis of the neck. 
Branches of the same hyphz project out free into the canal and upward 
toward the tip (Fig. 88, b). They are thin-walled, and react toward 
stains in a manner different from that of the other tissues. They are 
especially prominent at the tip of the neck. They are the periphyses, 
In cross section the wall of the body is seen to be composed of ten or 
twelve layers of flattened, heavy-walled cells, very compact and pseudo- 
parenchymatous (Fig. 88, a). Inside this there is a region of two or three 
layers of thin-walled cells, from the inner surface of the basal part of which 
the asci grow out into the cavity. 
Asct 
When the perithecium has become mature the entire cavity is filled 
with asci — the older, mature ones in the center, and younger ones around 
the walls. Mature asci are shown in Fig. 89, b, c, and d. They are 
broadly clavate or oblong, 
and each ascus contains 
eight spores in a matrix of 
epiplasm. The average 
size of one hundred and 
fifty asci measured by the 
writers was 51.2 by 
8.9 wu. The arrangement 
of the spores is irregularly 
uniseriate or sub-biseri- 
Fic. 89.— Ascospores and asci 
a, Three ascospores showing nuclei re fast 
b, Ascus with spores when dry ate; but there is little 
cand d, Asci after swelling in water uniformity in this arrange- 
ment, since two asci can hardly be found in which the spores 
are oriented alike. The wall of the ascus is delicate and hyaline, and for 
this reason is hard to make out in its entirety in unstained mounts. There 
is a thickened ring about the upper end of the lumen of the ascus which 
_is prominent and shows peculiar staining reactions. When the ascus is 
lying flat on the slide, the ring appears in optical section as two highly 
é refractive disks (Fig. 89, c). 
“‘Ascospores , 
The ascospores (Fig. 89, a) are oblong to oval with rounded or blunt- 
pointed ends, two-celled, constricted at the septa when mature, and on the 
average about 4.5 by 8.6 uw in size. The walls are thicker than those of 
the conidia; the septum is distinct and is composed of the same material 
as the walls. The spores are filled with dense homogeneous protoplasm; 
