64 THE VENOM OF HELODERMA. 



Rat U, 9S g. 



May 31, 12^ W^ injected subcutaneously 0.1 c.c. venom in 1 c.c. NaCl 0.85 per cent. 



13^ 30" lying on side; breathing rapid. 13^ 40^ lying on side; breathing slow. 



i^"" 45° reflexes present. i"" 00" reflexes absent. ^''.ZO"' better; able to move 



about. S^ 00^ lies on side; apparently dying; reflexes present. 2^ 50^ dead. 



Dog 1, 10 kg. 



May 31, S"" 14'" given subcutaneously 0.1 c.c. fresh venom. 3^ 26" vomited; urine 

 and feces voided. S"" SO" lying down; very sick. S'' (JO" vomited. June 

 1, died during forenoon. 

 Dog 3, 9700 g. 



May 31, 3^17" given subcutaneously 0.02 o.c. fresh venom. 3^ SO" lying down; 

 trembles; occasionally much twitching. 3^ 40" voids urine and feces. 

 Recovered. 

 Dog 3, 11 kg. 



May 31, 3^ 30" given 1.1. c.c. fresh venom subcutaneously. 3^ 45" vomited; urine 

 and feces voided. Very sick all afternoon. Died during night. 

 Cal 1, 3000 g. 



JuNEl, ^.g'" 70" give subcutaneously 0.4 c.c. fresh venom. 12^20"yora\is. 12^26" 

 pupils enormously dilated; vomits. i .2'' SO" very nervous; cries if touched; 

 breathing difficult. 2^ 00" breathing rapid, superficial, pupils still dilated. 

 Sick for some days, but recovered. 

 Cat 2, 3500 g. 



June 1, 12^ OS" given subcutaneously 0.09 c.c. of fresh venom. 12^ 25" pupils enor- 

 mously dilated. 12'' 40° breathing difficult. ^'OO" breathing rapid, super- 

 ficial, pupils dilated. Recovered. 

 Cal 3, 3000 g. 



June 1, 12^ 06" given subcutaneously 0.045 c.c. of fresh venom. 12^ 25" pupils enor- 

 mously dilated, vomits. 12^ 40" pupils contracting. Recovered. June 

 24 sick, killed; no lesions. 

 Cat 4, 3000 g. 



June 3, 2^ 12" given 0.9 c.c. of fresh venom subcutaneously. 2^ 20" A-iolent move- 

 ments of head from side to side, cries, pupils enormously dilated, eyes roll. 

 2'^ 35" more quiet; eyes still roll, but head movements stopped. 2^ 55" lies 

 quiet, very nervous; makes crying movements, but has nearly lost voice; breath- 

 ing difficult, audible. S'' 06" no longer able to hold up head; voice gone. 

 June 4 living; takes no food. June 6 dead. 

 Cat 7, 1500 g. 



June 8, 12^ 55" injected intraperitoneally 0.6 c.c. of fresh venom. 1^ 05" pupils 

 greatly dilated, losing voice. i"" 40" pupils contracted, voice gone; breathing 

 labored; tears secreted. Recovered. 

 Cat 9, 700 g. 



June 8, 2^ 25" injected intraperitoneally 0.7 c.c. of fresh venom. 2^ 35" very much 

 affected; pupils dilated; tears and saliva flowing; breathing forced. 5^00" 

 has remained in apparently dying condition all afternoon; breathing forced; 

 nearly paralyzed. 5^ SO" dead. 



CONDITION OF MOUSE'S EYE PRODUCED BY INJECTION OF 

 HELODERMA VENOM. 



We found that the injection of heloderma venom into mice caused fre- 

 quently a pecuHar condition of the eye. This condition did not appear as a 

 rule when mice were injected with a large quantity of venom — a quantity 

 which was lethal within a comparatively short time — but appeared usually 

 when quantities of venom had been injected which caused death after many 

 hours or several days. At times the injection of a sublethal dose of venom was 

 sufficient to produce this lesion. However, its appearance was not constant, 

 even after the injection of a lethal dose. 



The lesion was observed as early as from IJ to 6 hours after the injection 

 of the venom. At this time the eyeball appeared somewhat more prominent 

 than usual and the cornea showed a sHght opacity. The protrusion of the eye 

 and opacity of the cornea progressed fairly rapidly (there was, however, con- 



