224 APPLIED BACTERIOLOGY 



In squeezing the blood from the finger, the same effect may 

 be produced by the use of undue violence. 



Surgeon-Lieutenant Eogers speaks in favour of permanent 

 preparations, and finds Maynard's suggestion to fix unstained 

 specimens in the vapour of osmic acid (2 to 4 per cent.) 

 yields good results. He advises the spreading of a drop of 

 blood over the cover-glass with the edge of another, and 

 fixes the cover-glass down on the slide with four small 

 drops of paraffin. Wiirtz recommends staining the parasite 

 with a concentrated aqueous solution of methylene blue, or 

 with gentian violet or hsmatoxyline. He states that double 

 staining may be effected by acting successively on the dried 

 blood, first with a strong aqueous solution of eosin, which 

 stains the corpuscles pink, and then with a concentrated 

 aqueous solution of methyl blue, which colours the leuco- 

 cytes and the parasites blue. He considers the dark colour 

 of the blood in malarial fever to be due to the pigment 

 from the parasites being accumulated in the leucocytes. 



References to some Recent Literature. — A discussion on the 

 malarial parasite, before the Eoyal Medical and Chirurgical 

 Society, published in the British Medical Journal of Feb- 

 ruary 15, 1896, gives a synopsis of the views held by 

 various workers as to the life-history of the parasite. 



A paper by Dr. Maynard was published in the Indian 

 Medical Gazette of October, 1895, and papers by Surgeon- 

 Major Crombie and by Surgeon-Lieutenant Eogers appeared 

 in the same journal in February, 1896. 



The Goulstonian Lectures, by Dr. Patrick Manson, were 

 published in both the British Medical Journal and the Lancet 

 during March, 1896. 



