338 APPLIED BACTERIOLOGY 



small white points, which under a lens appear to have 

 well-defined outlines, and are yellow or yellowish-brown in 

 colour. 



Gelatine Tubes. — Liquefaction takes place at an early 

 date, and proceeds rapidly. The liquefaction occurs in 

 the form of a funnel-shaped tube, the liquefied gelatine 

 becoming very turbid. 



Agar-Agar. — A yellowish-white film is formed. 



Potatoes. — A yellowish white layer is formed at the 

 room-temperature. 



Bacillus riuorescens Liquefaciens. — This organism occurs 

 in water, air, soil, etc., and occurs more frequently than 

 any other form. It forms short bacilli, about 1 to 1'5 /x 

 long by 0"5 n broad. It occurs chiefly in pairs ; is very 

 motile. Cultural characters : 



Gelatine Plates. — Forms small white dots ; after about 

 forty-eight hours the gelatine becomes liquefied, and a 

 well-defined depression is formed. The whole of the 

 gelatine then very soon assumes a green fluorescence. 



Gelatine Tribes. — In the depth is a whitish growth, while 

 at the surface a funnel-shaped depression forms, and 

 finally the whole contents of the tube become liquefied and 

 of a fluorescent green colour, whilst a thick white deposit 

 is formed. 



Potatoes. — A brownish expansion is formed. 



Bacillus Fluorescens Non-Liquefaciens. — This organism is 

 found in water. It forms short fine bacilli with rounded 

 ends. It is strictly aerobic, and is not motile. Cultural 

 characters : 



Gelatine Plates. — The surface-colonies have a fern-like 

 appearance, with a mother-of-pearl-like opalescence. No 

 liquefaction of the gelatine takes place. 



Gelatine Tubes. — Produces a fluorescent film. 



