ENTOMOPHTHOREAE OF THE UNITED STATES. 169 



thread-like conidiophores, which may be seen produced from them abundantly in any 

 preparation, the ordinary form of secondary conidia being of rare occurrence. The pri- 

 mary conidia possess more than usually refractive, homogeneous contents, in which are 

 embedded numerous fine granules ; and the thickness of the spore wall is noticeable as 

 in the succeeding species, although in the present instance it is not indicated in figs. 110 

 -116. The growth of the fungus is never luxuriant, and each spherical hyphal body, 

 from its small size, can hardly give rise to more than one or two conidia. 



As already mentioned, I have taken this and the succeeding species {E. lageniformis) 

 as the types of a new sub-genus, Triplosporkmi, which will, I think, prove to have a 

 generic value when further information has been obtained concerning the development 

 of the last-named species. 



Empusa (Triplosporium) lageniformis nov. sp. 

 PL 16, figs. 141-160. 



Conidia slightly smoky, flask-shaped, with a truncate, hardly papillate base, rounded 

 apex and evenly granular contents; average measurements 20 x 35,a, maximum 30 x 38/i. 

 Conidiophores simple, or when young sometimes fasciculate or pseudodigitate ; terminat- 

 ing by a weak, tapeiing basidium. Cystidia not observed. Secondary conidia like the 

 primary, or almond-shaped and borne obliquely on capillary conidiophores. Mesting 

 spores unknown. Host attached to substratum by the insertion of its proboscis. 



Hosts. Hemiptera: usually aphides on Betula jMpulifolia. 



Habitat. Maine, Massachusetts, IS^oi'th Carolina. 



The discovery of the resting spores of this species is perhaps the most interesting fact 

 that remains to be observed among American Empusae, owing to its undoubted afiinity 

 with E. Fresenii. The species being of larger dimensions than H. Fresenii, we may 

 look for an instance of true conjugation which may be even more readily followed than 

 in the last mentioned form. I have found only scattered examples of this species in sin- 

 gle localities, although it is generally distributed in company with F. occidentalis, sub- 

 sequently described. Both forms occur, as far as I have observed them in ISTew England, 

 only upon a large Aphis generally found in abundance on the white birch in August 

 and September. I^umerous specimens examined contain no signs of resting spores, pro- 

 ducing only conidia which are similar to those of F. Fresenii, except as i-egards their 

 larger size and somewhat peculiar shape. The single specimen collected at CuUowhee 

 occurred on an Aphis infesting Solidago, so that it is probable that the disease could be 

 readily propagated among greenhouse aphides, for example, and thus afford a means of 

 observing its further development. 



Empusa (Entomophthora) Lampyridarum nov. sp. 

 PI. 17, figs. 161-172. 



Conidia regular, ovoid; slightly tapering towards the apex; with an abrupt, broad, 

 shghtly papillate base; contents granular, without large oil globules. Measurements 



