INOCULATING TUBES OF BOUILLON 15 
ts replaced in their respective tubes, and the loop flamed 
put aside. Label the freshly inoculated tube with the 
ie of the organism, source, and date. Stand it in a tray or 
and place it in the incubator.’ 
1. Inoculating tubes of agar. Ordinarily the agar ts 
ined before it is inoculated. In this case it is spoken of as 
ined or slant agar. Occasionally the agar is inoculated with- 
inclining it. Cultures made in this manner are spoken of as 
yor stick cultures. (@) Jnclined or slant agar. Stand a tube 
gar in a wire basket in a water bath and boil it until the agar 
quefied. (To save repeating this it is well to incline the agar 
everal tubes which can be kept for future use, but after the 
its have been made for a long time it is better to boil and 
ant them, especially if they are to be used for organisms 
ch do not grow well on a dry surface.) Lay the tube on a 
', the top resting on the side of the tray so that the surface of 
agar will be about 4 cm. long, and allow it to cool. In plac- 
the tubes the label should be up. When the agar has set, it 
zady for use. It is inoculated precisely as the bouillon, except 
t the loopful of culture is drawn over the inclined surface 
ead of being thrust into the medium as in the bouillon. Label 
. place it in the incubator with the inoculated bouillon tube. 
the following day there should be a grayish-white growth on 
surface of the agar covered by the,loop. This is an agar 
cure of B. colt. (6) Stab cultures. These are made with a 
tinum needle in the uninclined agar. The impregnated 
dle is pushed down through the center of the agar. In all 
er respects this culture is made like the slant-agar culture. 
For illustrations and descriptions of different kinds of incubators, 
text-books. It is desirable to note especially the various burners 
thermoregulators employed to heat and regulate the temperature 
he incubators. Considerable information may also be acquired by 
fully looking through the catalogues of manufacturers and dealers 
acteriologic apparatus. Copies of some of these will be found on 
reference bookshelves, 
