BACTERIUM MALLEI 77 
EXERCISE XXVI 
BACTERIUM MALLEI 
115. This organism grows most characteristically on potato 
and somewhat feebly in the other media heretofore used. It 
develops readily on acid agar and on acid glycerin agar and 
in acid glycerin bouillon. For this reason it is not inoculated 
into all the media. In diagnosing glanders it is customary 
to inoculate guinea pigs with the suspected material: From 
the lesions in these animals, if the disease develops, pure cul- 
tures can usually be obtained. It can be identified by its 
morphologic and cultural characters. 
116. Work for this exercise. Inoculate a tube of potato, 
one of agar, one of acid agar, one of acid glycerin agar, one of 
glucose agar, one of bouillon, and one of acid glycerin bouillon 
from a culturé of Bact. maillei (furnished). (The special media 
here introduced will be furnished by the instructor.) 
Stain cover-glass preparationsg(furnished) made from the 
lesions in guinea pigs which were inoculated with this organ- 
ism. Stain one with alkaline methylene blue and one with 
carbol fuchsin. Note especially the morphology and the extent 
to which the organisms take the stain. 
Reéxamine the cultures of Bact. diphtherie and complete 
the notes on the same. 
