82 LABORATORY BACTERIOLOGY 
EXERCISE XXX 
BACTERIUM PULLORUM 
123. Bact. pullorum is the cause of a severe diarrhea (bac- 
illary white diarrhea) of young chickens. It has recently been 
found to cause a disease usually fatal in adult fowls. It differs 
very little from Bact. sanguinarium. It was originally described 
by Rettger. 
124. Work for this exercise. Examine and describe the 
cultures of Bact. pullorum made in the last exercise, 
Examine microscopically in both stained and hanging-drop 
preparations the bouillon and agar cultures. 
Note particularly the action of this organism on milk and 
the sugar bouillons. : 
Make the indol test (§ 127) with the culture in sugar-free 
bouillon of the organism of the septicemia group studied in 
the last exercise. 
Inoculate a tube of each of the media in groups A and D 
from a culture of 2B. cof (furnished) for study at the next 
exercise. 
