BACILLUS TETANI gI 
cultures made directly from the lesions are usually impure. 
I have found that pure cultures may often be obtained by in- 
oculating a guinea pig with the pus or exudate from the local 
lesion and making cultures from the local lesions in the guinea 
pig, the juices of the body having destroyed the saprophytic 
bacteria which were present in the first material. 
Kitasato has recommended a procedure which is reported 
to be fairly successful. It is to inoculate a tube of agar with 
tissue from the local lesion, and after it has grown for from 24 
to 48 hours at a temperature of 37°C. heat the tube to 80°C., 
which kills all.the other bacteria but does not destroy the 
tetanus spores. From this culture anaérobic cultures are 
prepared. 
