ISOLATING BACTERIA FROM ANIMAL TISSUES 95 
EXERCISE XXXVIII 
ISOLATING BACTERIA FROM ANIMAL TISSUES 
145. In making a bacteriologic investigation into the cause 
of death in an animal or man it is necessary to make cultures 
from the various organs and from the blood to find whether or 
not there are any pathogenic or other bacteria present. This 
necessitates a knowledge of making cultures from animal tis- 
sues. In this exercise an experimental animal (rabbit or guinea 
pig) which has died from some bacterial disease will be pro- 
vided. The purpose of this examination is to find out what 
that disease is. To save animals, each student will make cul- 
tures from but one organ. From time to time during the course 
opportunity will be afforded for making cultures from variously 
diseased animal tissues. Each student will be given opportunity 
at some time during the course to inoculate one or more animals. 
146. Work for this exercise. The experimental animal fur- 
nished will be tied out on a post-mortem tray and the viscera 
exposed. (Directions for the post-mortem examination will be 
given in the course in pathology.) 
Inoculate a tube of bouillon, a tube of agar, and a fermenta- 
tion tube of glucose bouillon from either the liver, spleen, or 
kidney. (In ‘an actual investigation of an unknown disease, 
cultures should ‘ke made from all of the organs, and from the 
blood and the lymphatic glands.) 
Make a series of 3 agar plate cultures from the same ereany 
Make and examine 2 cover-glass preparations from the 
organ from which the ee were made. Stain one with 
alkaline methylene blue and gne with carbol fuchsin. (It is 
sometimes necessary to fix pieces of the tissue in alcohol or 
in some other fixing fluid for sectioning and staining, pre- 
paratory to studying them.) 
Preserve one of the cover-glass preparations. 
