96 LABORATORY BACTERIOLOGY 
147. Making cultures from animal tissues. Heat a platinum 
spatula to a white heat in a gas flame and scorch the surface 
of the organ. Flame a pair of fine forceps, tear an opening 
through the scorched surface, and crush a bit of the tissue 
underneath it. With the platinum loop take up a loopful of 
the crushed tissue and inoculate the media with it. It is also 
desirable to inoculate a tube of slant agar with the needle by 
drawing it over the surface of the medium after charging it 
with tissue. In making plate cultures use a loopful of the 
crushed tissue for the first tube. The quantity of the tissue 
necessary to give a desired number of colonies cannot be 
anticipated, although experience in working with different 
organisms in animals renders one able to approximate the © 
amount required. 
