DIAGNOSIS OF RABIES 129 
(7) Wash in tap water, dry, and dehydrate for from 5 to 20 
seconds in absolute alcohol. 
(8) Clear in xylene. 
(9) Mount in balsam.? 
The Negri bodies appear as round or oval purplish- -red 
bodies lying outside of the nucleus within the protoplasm of 
the large nerve cells. 
The following procedure is recommended in diagnosing 
rabies from suspected animals sent to laboratories for this 
purpose : 
1. Examine for Negri bodies, and if they are found, report 
the disease as positive. 
2. If Negri bodies cannot be found, examine the Gasserian 
ganglia for cellular lesions. 
3. If the results from the examination for Negri bodies and 
ganglion changes are negative, rabbits should be inoculated in 
those cases where a late diagnosis will be of value. Other- 
wise, animal inoculations should be omitted. 
In suspected rabies the entire head of the animal should be 
packed in ice and shipped. However, the entire brain may 
be removed and placed in glycerin. It is very desirable that in 
the latter case the brain be removed without laceration. 
1 The smear may be examined without balsam and a cover glass. 
