ae 
Carbol fuchsin, preparation of, 26 
staining with, 24 
Carbolic gentian violet, prepara- 
tion of, 27 
Carbolic thionine blue, prepara- 
tion of, 27 
Chester’s terminology, 20, 34 
Cleaning mixture, formula for, 2 
Colonies, Chester’s terminology 
for, 34 
estimating number of, 33 
subcultures from, 34 
Complement-fixation test for 
glanders, 123 
Cover glasses, cleaning, 2 
for flagella stain, 3 
Cover-glass preparations, making 
of, 23 
from milk cultures, 51 
from the mouth, 93 
from pus, 99 
from sputum, 80 
from tissues, 72 
staining bacteria in, 24 
Culture apparatus, cleaning, 3 
Culture tubes, labeling, 10 
sealing, 16 
Cultures, anaérobic, 88 
cover-glass preparations of, 23 
determination of viscidity of, 18 
examination of, 17, 48 
from animal tissue, 96 
hanging-drop preparations from, 
19 
identifying bacteria from, 94 
labeling, 10 
macroscopic examination of, 17 
microscopic examination of, 19 
plate, 29 
reaction of liquid, 18 
sealing, 16 
study of, on special media, 49 
viscidity of, 18 
Diagnosis, animal inoculation for, 
114 
of actinomycosis, 115 
of anthrax, 116 
of black leg, 119 
of dourine, 135 
LABORATORY BACTERIOLOGY 
of glanders, 120 
of hog cholera, 124 
of non-specific lesions, 138 
of protozoan diseases, 135 
of rabies, 125 
sending tissues for laboratory, 
112 
of surra, 135 
of swine plague, 130 
of Texas fever, 135 
of tuberculosis, 131 
Disinfectants, determining effi- 
ciency of, 103 
Dourine, diagnosis of, 135 
Drawings of bacteria, 57 
Dunham’s peptone solution, 84 
Egg medium, preparation of, 43 
Exudates, microscopic examina- 
tion of, 98 
Fermentation tubes, cleaning, 2 
for anaérobic bacteria, 89 
use of, 48, 49, 50 
Filar micrometer, 145 
Finger nails, bacteriologic exami- 
nation of, 100 
Flagella, 58 
staining of, 60 
Johnston and Mack’s method, 
60 
Loeffler’s method, 62 
Flasks, cleaning, 2 
plugging, § 
Fontes method, 65 
Fungi, examination of, 107 
Gabbett’s method, 64, 79 
Gas, production of, by bacteria, 50 
quantity produced, 50 
ratio of CO, to H, 50 
Gelatin, 7, 11 
inoculating tubes of, 16 
Preparation of nutrient, II 
Genera, identifying, 66 
Glanders, diagnosis of, 120 
Glassware, cleaning, 1-3 
sterilizing, 6 
Glucose agar, inoculating, 46 
preparation of, 41 
