INDEX 
Glucose bouillon, preparation of, 
41 
Glycerin agar, preparation of, 41 
Gram’s method of staining, 28 
Hanging-drop preparations, 19 
Herman’s method of staining, 65 
Hog cholera, diagnosis of, 124 
Identifying bacteria, from animal 
tissues, 97 
from cultures, 94 
species, 66 
Indol test, 84 
Inoculating media, agar, 15 
bouillon, 14 
gelatin, 16 
glucose agar, 46 
Isolating &. coi from intestine, 85 
B. tetant, 90 
Isolating bacteria from animal tis- 
sue, 95 
Jeffers plate, 33, 148 
Kitasato’s method, 91 
Labeling cover-glass preparations, 
25 
Labeling media and cultures, 10 
Laboratory notes, 4 
Lactose bouillon, preparation of, 
41 
Lesions of hog cholera, 124 
Liborius’ method, anaérobic cul- 
tures, 89 
Living bacteria, microscopic ex- 
amination of, 19 
M‘Fadyean’s stain for anthrax, 
116 
Media, degree of reaction of, 142, 
143 
effect of bacteria upon, 48 
grouping of, 43, 44, 45 
labeling, 10 
neutralization of, 142 
preparation of, 39 
reaction of, 139, 141 
short method of sterilizing, 9 
153 
special, 39 
use of, containing sugars, 46 
Methylene blue, alkaline, prepara- 
tion of, 26 
staining with, 24 
Metric system, 149 
Micrococcus, morphology of, 55 
pyogenes, 70, 71 
Micrometer, eye-piece, 144 
measurements, 146 
ocular, 144 
valuation of, 145, 146 
varying valuation of, 147 
Micrometry, 144 
Microscopic examination, of exu- 
dates, 98 
of pus, 98 
of stained bacteria, 25 
Migula’s classification of bacteria, 
5 
Milk, Pvetailata preparations 
from, 51 
preparation of litmus, for cul- 
ture media, 40 
Mordants, preparation of, 61 
Morphology, of bacillus, 57 
of bacterium, 57 
of micrococcus, 55 
of sarcina, 55 - 
of spirillum, 57 
of streptococcus, 55 
Negri bodies, 128 
Neisser’s method of staining, 76 
Nitrate bouillon, preparation of, 
43 
Non-specific lesions, diagnosis of, 
138 
Notes, laboratory, 4 
Pasteurella, 81 
Pasteurizing milk, 105 
Petri dishes, cleaning, 2 
Plate cultures, agar, 30 
examination of, 32 
gelatin, 30 
use of, 29 
Plugging tubes and flasks, 5 
Potato, preparation of, for media, 
40 
