154 
Protozoa, diagnosis of diseases 
caused by, 135 
examination of, 107 
Pseudomonas fluorescens ligue- 
JSaciens, 69, 70, 71 
Pseud S pyocy Sy 70, 71 
Pus, cover-glass preparations from, 
99 
examination of, 98 
Rabies, diagnosis of, 125 
Reagent bottles, cleaning, 2 
Roll cultures, Esmarch, 31 
examination of, 32 
Saccharose bouillon, preparation 
of, 41 
Saprophytic bacteria, 66 
Sarcina, morphology of, 55 
Sealing culture tubes, 16 
Sections, study of bacteria in, 97 
Skin, bacteriologic examination of, 
100 
Slides, cleaning, 2 
Spirillum, morphology of, 57 
Spores, 58 
staining of, 59 
Sputum, 79 
cover-glass preparation of, 80 
Staining blood films for animal 
parasites, 136 
Staining cover-glass preparations, 
24 
Staining flagella, 58, 60 
Staining spores, 58, 59 
Staining solutions, 25 
alkaline methylene blue, 26 
LABORATORY BACTERIOLOGY 
aniline gentian violet, 27 
aqueous solutions, 27: 
carbol fuchsin, 26 
carbolic gentian violet, 27 
carbolic thionine blue, 27 
formule for, 26 
Gram’s solution, 28 
Sudan III, 65 
Staphylococcus. See micrococeu 
Sterilizing milk, 105 
Streptococcus, morphology of, 
pyogenes, 70 
Subcultures, making of, 34 
Sugar-free bouillon, preparation « 
41 
Sugars, use of media containin 
4 
Surra, diagnosis of, 135 
Swine plague, diagnosis 
130 
Temperature, Centigrade, 149 
Fahrenheit, 149 
Test tubes, cleaning, 1, 2 
plugging, 5 
Texas fever, diagnosis of, 135 
Thermal death point, determin 
tion of, for bacteria, 1o1 
Tubercule bacteria, cultivation 
131 
staining of, 63, 64, 65 
Tuberculosis, diagnosis of, 131 
Viscidity, determination of, 18 
Wolffhiigel’s counting apparatu 
33 
