72 BACTERIOLOGICAL DIAGNOSIS. 
A culture which presents these cultural and morpho- 
logical appearances may be considered to be one of 
anthrax with almost absolute certainty, though other 
tests (notably animal tests) would be applied in a 
laboratory. 
TUBERCLE. 
The diagnosis of tuberculosis by bacteriological 
methods (in the case of most morbid exudates) is within 
the reach of every practitioner; cultural methods are 
not used and the recognition of the bacillus is rapid, 
easy, and certain. 
The bacilli may be sought for in sputum, urine, pus, 
feeces, or any other morbid material. We will first 
describe the method of staining which should be 
adopted, then the characters on which the recognition 
of the bacillus depends, and lastly the methods by 
which the films are prepared from the various materials. 
STAINING THE TUBERCLE BacILuus. 
Requisites.—1. Slides, cover-glasses, forceps, and bal- 
sam. 
2. A Bunsen burner or spirit lamp. 
3. Dilute sulphuric acid—about 20 per cent.—con- 
tained in a wide-mouthed bottle or in a jar. This must 
be large enough to admit a slide but not large enough 
to permit it to fall down to the bottom. 
4. Carbol-fuchsin. 
