204 BACTERIOLOGICAL DIAGNOSIS. 
6. Methylene blue for three or four minutes. Some 
of the stain comes out in the alcohol, so that the section 
must be stained more deeply than will be required 
ultimately. 
8. Rinse off the blue stain in water, and then remove 
the greater part of the latter with blotting-paper; this 
is to render the dehydration more rapid. 
g. Absolute alcohol—two lots in rapid succession. 
10. Xylol. 
11. Balsam and cover-glass. 
