12 Wisconsin Academy of Sciences, Arts, and Letters. 



ness had occurred. In the remaining three trees traces of starch 

 were present which soon disappeared. The radial growth 

 ranged from 25% to normal. The tree defoliated June 15 was 

 dead by October and the one defoliated in August by the follow- 

 ing May. 



The stems of the first four and of some untreated young pines 

 were cut in 15 to 30 em. pieces in October and by December the 

 bark on the treated-tree pieces was found to have loosened espe- 

 cially where considerable radial growth had occurred. The bark 

 had split and was shrunken both in length and circumference; 

 while that on the pieces from untreated trees adhered firmly to 

 the wood. In December pieces were also cut from the branches 

 of the last treated tree, the bark of which had lost its turgidity 

 after the operation but regained it again. A discolored circle 

 was found in the cambial region. Groups of undifferentiated 

 wood cells had been ruptured or broken down and were discol- 

 ored. 



In his researches on the reserve food of trees du Sablon 26 

 found that the carbohydrate content underwent farily definite 

 seasonal changes which apparently occurred irrespective of the 

 weather. On March 17 the roots of pear trees contained much 

 more sugar and very much more starch than the stems and the 

 total carbohydrate content of roots was also higher. In stems 

 of chestnut trees the carbohydrate content reached a maximum 

 in October and a minimum in May, while in roots the maximum 

 came in September and the minimum in May. In case of quince 

 the maximum in both root and stem was found in January with 

 a minimum in stems in May and in roots in June. In peach the 

 minimum in both root and stem came in May and the maximum 

 in the stem in July and in roots in November. In willow both 

 stem and roots were found to have a minimum of carbohydrates 

 in April and a maximum in October, but both the maximum and 

 minimum were more extreme in the roots. In the case of rasp- 

 berry bushes the roots had a minimum in April and a maximum 

 in October, while in the biennial stems a high carbohydrate eon- 

 tent was maintained during the first summer with a maximum in 

 October, followed by a slight depression and subsequently a les- 

 ser maximum in the second April. Afterwards a fairly constant 



18 du Sablon, Leclerc. Recherches physiologiques sur les matures de 

 reserves des arbres. Rev. Gen. Bot. 16:339-68; 386-401. 1904. 



