51 



THE ORGANISM IS A BACILLUS. 



THERE IS NO GROWTH ON GELATINE AT 22° C. 



Bacillus tuberculosis (Koch), from sputura and other secretions in 

 eases of tubercle ; rod-like bacilli, sUghtly curved, from 1 to 3 /t 

 long; when stained they have a beaded appearance; is said 

 to form oval spores (Sohenk) ; stains well by Gram, also by 

 Ziehl-Neelsen's carbol-fuchsin solution. Extremely difficult to 

 cultivate directly from sputum on account of the numerous 

 other organisms present ; best obtained by secondary culture 

 through inoculation ; will grow on blood serum or glycerine 

 agar at 37° C. ; on serum the colonies appear in from ten to 

 twenty-one days as minute dry white scales. 



Bacillus mallei — bacillus of glanders (Loffler and Schiitz), bacilU 

 with round ends, straight or shghtly curved, shorter and thicker 

 than tubercle ; stains best with methylene blue ; is decolorized 

 by acids and Gram solution ; will grow on most media at 37° C. 



Bacillus of diphtheria (Klebs-Loffler), from diphtheritic false mem- 

 brane ; straight or slightly curved non-motUe rods with rounded 

 ends, 2 to 3 /t in length ; stains irregularly, the refractive 

 portion of the rods staining most deeply ; stains by Gram ; 

 is best grown on blood serum at 37° C. ; the colonies appear in 

 24 hours as grey semi -translucent discs, thick in the centre, 

 with a thin irregular border. 



Bacillus septicus sputigenus (Diplococcus pneumonise), from pneu- 

 monic sputum and various purulent secretions ; common even 

 in healthy saliva ; grows in various media at 37° C. (see 

 Diplococcus pneumonise, p. 27). 



Bacillus of influenza (Pfeiffer), from purulent bronchial secretion in 

 epidemic influenza ; has also been found in the blood in these 

 cases (Canon) ; an extremely small, non-motile bacUlus, re- 

 sembling a diplococcus ; sometimes in short chains ; does not 

 stain by Gram ; is best grown on glycerine agar at 37° C* 



* Czenzynke'a stain for the influenza bacillus in the blood : Concentrated 

 aqueous solution of methylene blue, 40 grammes ; J per cent, solution of 

 eosin in 70 per cent, alcohol, 20 grammes ; distilled water, 40 grammes. 

 Cover glasses to be left in the stain from four to six hours at 37° C. 



