10 



THE OEGANISM IS A MICEOCOCCUS. 



THE GELATINE IS LIQUEFIED. 



THE COLONIES ABE WHITE. 



THE COCCI AEE PEINCIPALLY IN CHAINS. 



Micrococcus ureae lig.uefacieiis (Pliigge), from ammoniacal urine ; 



spherical cocci 1'25 to 2 /* in diameter ; a yellowish-white deposit 



is formed in the liquefied gelatine. 

 Micrococcus Freudenreichi, from milk which had undergone viscous 



fermentation, cocci having a diameter of 2 /i, singly or in chains. 

 Streptococcus lic[uefaciens (Sternberg), from intestinal secretions ; 



oval cocci 0-4 /j. in diameter, in pairs or chains. 

 Streptococcus of Manneberg, from mine in acute Bright's disease ; 



cocci 0'9 ft in diameter, in pairs or short chains ; stains .by 



Gram.* ' 



Streptococcus coli gracilis (Bscherich), from the faeces of children fed 



on a flesh diet ; cocci -2 to "4 /i in diameter in short S-shaped 



chains. 

 Streptococcus septicus liquefaciens (Babfes), from blood in a case 



of septicaemia following scarlet fever ; cocci 0'3 jn in dianaeter, in 



pairs or short chains ; stains by Gram. 



* Frequent allusion is made in the text to Gram's method of staining, as 

 an aid to the diagnosis of a great mmiber of micro-organisms ; the method of 

 procedure is therefore given. The section or fixed cover-glass preparation is 

 gently warmed in EhrUoh's aniline-gentian-violet for 15 minutes, then placed 

 in Gram's iodine solution (iodine, 1 part ; potassium iodide, 2 parts ; water, 

 300 parts) for 2 minutes, and transferred to absolute alcohol untU decolorized. 

 The bacteria are stained by the gentian violet, and the tissue can be counter- 

 stained with eosin, picrocarmine, etc. 



