INTEBACTIONS BETWEEN VENOM AND ANTIVENIN 



251 



The rise of the minimal hsemolyzing doses through [the fractions of the 



serum necessary for complete neutralization was as follows: 



Table 27. 



0.00 1 gm. venom alone 0.000005 gm. 



+0.1 c.c. serum 0.000025 



+0.2 0.00005 



+0.4 0.00008 



+0.6 0.00017 



+0.8 0.0005 



In another way the above results can be summarized as follows: 

 0.001 gm. of venom contains 10,000 -=- 5 = 2,000 minimal hsemolyzing 

 doses. With 0.1 c.c. of serum necessary for a complete neutralization it now 

 contains 10,000 -h 25 = 400 minimal hsemolyzing doses. If we arbitrarily 

 call the number of combining equivalents in 0.001 gm. of venom 130, the above 

 fraction neutralized 1,600 minimal hsemolyzing doses per 10 equivalents. On 

 the theory of simple neutralization we should have neutralized 2,000 -5- 13 = 

 153 minimal hsemolyzing doses. Myers gives the following calculations: 



Table 28. 



It will be seen at once that the interaction of the cobralysin and its antilysin 

 is very different from that of a single neutralization, say of a strong acid by a 

 strong base. If we explain this reaction according to the toxoid theory of 

 Ehrlich, as was done by Myers, the results may be represented as in figure 1. 



0v6 0-7 0.8 



Fro. „. 



