INVESTIGATIONS PERTAINING TO TEXAS BEEKEEPING. 
29 
Table II.—Results of Fumigating Infested Combs with Carbon Bisulfide. 
Amount of Liquid 
Stage of Carbon Bisulfide Time of Effect. Remarks. 
Bee-moth. Used ey Gabic Confinement. 
‘oot. 
Moth....... One-half ounce....... 15 minutes... .;Killed..... 
Moth ‘T'wo-thirds ounce... .|20 minutes.“ ./Killed...//,The moth was unable to 
: pe partes = sl ig 
r r er being confined. 
Moth....... Three-eighths ounce |20 minutes... ./Killed..... The moth Was unable to 
walk within 10 minutes. 
Not ill ane bisulfide 
evaporated. 
Matias cay One-fourth ounce... .. 20 minutes... ./Killed..... The moth was dead before 
all the bisulfide evap- 
. orated. 
Pupae....... One-sixth ounce...... 24 hours....... Killed..... Several larvae in cocoons 
were also killed. 
Pupae....... One-fourth ounce..... 24 hours....... Killed..... Several larvae in cocoons 
2 were also killed. 
Pupae....... Three-eighths ounce. .{24 hours....... Killed..... Several larvae: in cocoons 
were also killed. 
Pupae....... One-half ounce....... 24 hours....... Killed..... Several larvae in cocoons 
, were also killed. 
Larvae...... One-eighth ounce..... 24 hours....... Killed..... Some died in 1 hour, in the 
(in cocoons) cocoon. 
MVOC nc 25. One-fourth ounce..... 24 hours....... Killed..... Some died in 134 hours in 
Gin cocoons) | the cocoon. 
Larvae...... Five-eighths ounce... ./24 hours ...... Killed..... 
(in cocoons) 
arvae...... One-sixth ounce...... 24 hours....... Killed..... Larvae were 10 days old 
(exposed) * : and well protected. 
arvae...... One-eighth ounce..... OA TOURS ey gx Killed..... These were 5 days old and 
(exposed) * . well protected in webs. 
arvae...... One-eighth ounce..... 24 hours....... Killed..... These were 25 days old and 
(exposed) * protected. 
arvae...... One-fourth ounce..... 24 hours....... Killed..... These were 20 days old and 
(exposed) * i fairly well protected. 
arvae...... One-fourth ounce..... 24 hours....... Killed..... These were 20 days old and 
(exposed) * exposed. 
arvae...... One-fourth ounce..... 24 hours....... Killed..... These were 12 days old and 
(exposed) * exposed. 
arvae...... One-half ounce....... 24 hours....... Killed..... These were 15 days old and 
(exposed) * fairly well protected. | 
arvae...... Three-fourths ounce. ./24 hours....... Killed..... Eggs were present which 
(exposed) * atched afterwards. 
Larvae...... Three-fourths ounce. ./24 hours....... Killed..... Eggs were present; hatched 
(exposed) * afterwards. 
arvae...... One ounce........... 24 hours....... Killed..... Eggs were present; hatched 
(exposed) * afterwards. ; 
arvae...... One ounce........... 24 hours....... Killed..... Eggs were present; hatched 
(exposed) * afterwards. 
arvae...... One ounce........... 24 hours....... Killed..... : 
(exposed) * 
In all the experiments conducted, the eggs of the bee-moth were un- 
injured by the fumes of carbon bisulfide. It is possible that in cases of 
extremely large doses the eggs may be injured. : 
A number of experiments were conducted to determine the effect of 
the fumes of carbon bisulfide upon the larve. Comb containing larve 
of various ages and different degrees of protection were fumigated. Many 
experiments were made with the larve in cocoons, and these showed that 
carbon bisulfide is very effective. 
The larve which are hardest to kill 
are those about three-fourths grown and well protected in a mass of webs 
and refuse. Ordinarily the larve succumb to the average dose of carbon 
bisulfide in a comparatively short time. The outcome of the experiments 
demonstrated the effectiveness of carbon bisulfide for the destruction of 
the larve. 
Several experiments were conducted to determine the effect of carbon 
*These larve were feeding in empty combs. 
