37 



1 66. To another portion add, very cautiously, some strong 

 sulphuric acid. If a cherry red color results at the junction 

 of the fluids it denotes the presence of cholesterin. Make 

 a control test by putting a little ether in a test-tube and 

 adding some strong sulphuric acid. No red color should 

 appear. 



167. Saline extract of Liver. (Epithelial Cells. ) Divide 

 the fluid into three portions. 



168. To one portion apply the xanthoproteic test. 



169. Faintly acidify another portion with dilute acetic 

 acid and boil. If coagulated it indicates native albumin or 

 globulin or both. 



170. Saturate the third portion with magnesium sulphate. 

 A precipitate should occur of a proteid character. The 

 proteid may be globulin and partly nucleo-albumin. 



171. Filter the mixture obtained in 170. Boil the filtrate, 

 lyittle or no coagulation may occur, indicating little or no 

 albumin in the filtrate. 



172. Mince a small piece of liver from an animal which 

 has been dead for 24 hours. Boil the liver either in water 

 or a saturated solution of sodium sulphate. Filter. The 

 filtrate should not be opalescent. 



173. Test the reaction of the filtrate to litmus paper. 



174. Neutralize a portion of the filtrate with a little so- 

 dium carbonate and filter ; then test with iodine for glyco- 

 gen. If there is no deep brown mahogany color, glycogen 

 is absent. 



175. Test for grape sugar, 50-52. After death the gly- 

 cogen is tran,sformed into grape sugar, unless precautions 

 be taken to prevent this transformation. 



176. Saline extract of muscle. The reaction of perfectly 

 fresh muscle to litmus is of an alkaline character. That of 

 butchers' meat is acid due to the formation of sarcolactic 

 acid. A muscle tetanized for a long time becomes acid. 



177. Pour a few drops of the saline extract into a large 



