38 



quantity of water. Observe the milky deposit of myosino- 

 gen. The precipitate is redissolved by adding a strong so- 

 lution of common salt. 



178. Test the coagulating point of another portion of the 

 extract. Four proteids are coagulated by heat, each re- 

 spectively at 47°, 56°, 63°, and 73" C. an albumose being 

 left in solution. The fluid is acid in reaction. Filter off 

 the coagula as they are formed. 



179. Saturate the final filtrate with sodium chloride. 

 The myosinogen is precipitated. 



180. Collect some of the precipitate of 179 and dissolve 

 it in a weak solution of sodium chloride and test for proteid 

 reactions. Xanthoproteic and Millon's tests. 



181. Make a solution of I^iebig's extract of meat. Test 

 a small portion of it for proteids. 



182. Test another portion for glycogen by adding iodine 

 solution ; a red-brown or port wine color indicates glycogen. 

 Make another test by adding a little basic lead acetate. 



183. Test another portion for kreatinin by Weyl's test. 

 Add a very dilute solution of sodium nitro-pru.sside, and 

 very cautiouisly some caustic soda ; an evanescent ruby-red 

 color, pas.sing into a straw color, indicates kreatinin. 



XV. 



184. Blood. Fresh blood may be obtained and defibrin- 

 ated at a slaughter house, and a few drops of formalin added 

 to it will prevent putrefaction for a long time. It is better, 

 however, when possible, to obtain the blood by bleeding an 

 animal. After the dog, or any other animal of convenient 

 size, has been anesthetized, the carotid or femoral artery is 

 exposed and isolated from, surrounding parts for an inch or 

 two of its length, and a clamp or ligature applied to the 

 proximal portion of the artery, i. e., as far as possible toward 



