43 



213- Detection of paraglobulin (fibrinoplastin, or serum- 

 globulin). Pass some COj through a beaker of dilute 

 serum for 20 minutes or more. (The CO2 may be gen- 

 erated by the action of dilute hydrochloric acid upon small 

 pieces of marble in a jar and the gas conveyed to the 

 beaker.) I^et the precipitate settle. It is paraglobulin. 

 Decant and, after washing with water, dis.solve some of it 

 in a little dilute saline solution, use Piowtrowski's test and 

 prove it a proteid. 



214. Take equal quantities of blood and ether in a test- 

 tube. Shake thoroughly and let the ether separate. Then 

 pour the ether into a watch-gla.ss or evaporating dish and 

 when evaporated examine for globules of fat. 



215. Evaporate a little blood to dryness in a crucible or 

 evaporating dish. Raise the temperature to red heat to con- 

 vert the blood to ash. When cool add a little nitric acid, 

 heat, dilute with water and filter. Make the following 

 tests with the filtrate : 



216. To a small portion of the filtrate add a little sulpho- 

 cyanide of potassium. A red color indicates iron. 



217. To another portion add a little ammonium molybdate 

 solution. A yellow precipitate, after allowing the mixture 

 to .stand for some time, indicates pho.sphates. 



218. To another portion add a little silver nitrate solution. 

 A white, cloudy precipitate indicates chlorides. 



219. Examination of blood with a spectroscope. With a 

 small direct vision spectroscope focus on the sky or bright 

 light until the spectrum .«hows clearly. Narrow the 

 slit until the spectrum is as distinct as it can be made. Hold 

 the spectro.scope so that the red is at the left of the field. 

 Dip a wire into some water, and then into .some salt or sodi- 

 um carbonate, and hold it in a flame of a fish-tail burner. 

 Note the change in the spectrum. 



220. Arrange the apparatus with the aid of a demonstra- 

 tor, so that the spectroscope, gas-flame, and substance to be 



