28 



a portion of the contents of tubes No. i and 2 for indol and 

 tyrosin tests later, (131-132). 



118. Take three more tubes for each of the three series, 

 add starch mucilage to each test-tube, about one-sixth fulL 



119. Half fill three test tubes with \cJo sodium carbonate- 

 and add 30 drops of the pancreatic extract from the pig, ox 

 and dog to their respective tubes. 



120. Half fill one tube with 1% sodium carbonate andi 

 add 30 drops of bile. 



121. Half fill three tubes with 1% sodium carbonate and 

 add to each tube 30 drops of the respective extracts and 30- 

 drops of bile. 



Place these tubes in the water bath at 40° C. Test small 

 portions of each every minute with iodine and note in which 

 one the starch first disappears. 



122. Test Nos. 119, 120 and 121 for sugar by Trommer's. 

 test, (50). 



XI. 



123. Prepare four test-tubes as follows, labeling them in 

 order i, 2, 3, 4, and adding a bit of fibrin to each tube. 

 To No. I add 30 drops of the pancreatic extract from the 

 pig, and some distilled water ; to No. 2, the .same amount of 

 the pig's pancreatic extract and excess of 0.2% hydrochloric 

 acid ; to No. 3, some 1% .sodium carbonate alone; for No.. 

 4, put 30 drops of the pig's pancreatic extract into a 

 separate test tube, add a little of the i % sodium carbonate 

 and boil, and then add the fibrin. Put all of the test tubes- 

 in the water-bath at 40° C. After a few hours examine them 

 and explain the results. 



124. Emulsion Experiment. Shake up a few drops of 

 olive oil in a test-tube with some artificial pancreatic juice. 

 Place the mixture for a few minutes in the bath at 40° C, 

 and shake again, compare the results before and after 

 warming. 



